Expediting Antibody Discovery with a Cell and Bead Multiplexed
Por um escritor misterioso
Last updated 24 abril 2025

A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.

Luminex Discovery Assays R&D Systems, a Bio-Techne Brand

iQue® Mouse IgG Type and Titer Assay - Intellicyt

What Are Immunoassays Bead-Based Immunoassays

Multiplexed Immunoassays: Small Samples, Big Pictures

A Microfluidic Chip for Screening and Sequencing of Monoclonal

Using Multiplexed Screening for Advanced Therapeutic Antibody

Characterization of antibodies from the established hybridomas by

ELISA in the multiplex era: Potentials and pitfalls – topic of

Antibody Screening Solutions Made Easy - 6

An immunogenic cell injury module for the single-cell multiplexed

Human immunocompetent Organ-on-Chip platforms allow safety

Multiplex Technology for Biomarker Immunoassays

Single B-cell Antibody Development Service
Recomendado para você
-
Shotgun King IGG Games The Final Checkmate v1.39 Download24 abril 2025
-
PDF) Streptococcus pyogenes evades adaptive immunity through24 abril 2025
-
Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies24 abril 2025
-
Systemic IgG repertoire as a biomarker for translocating gut24 abril 2025
-
Gut microbiota‐derived synbiotic formula (SIM01) as a novel24 abril 2025
-
Dynamic interactome of the MHC I peptide loading complex in human24 abril 2025
-
Reinvestigating the Coughing Rat Model of Pertussis To Understand24 abril 2025
-
Delayed booster dosing improves human antigen-specific Ig and B24 abril 2025
-
Bigg TV24 abril 2025
-
Reading IGN's top 100 RPG list, when I noticed something odd24 abril 2025
você pode gostar
-
Cheap Bandage Cyber Y2k Sexy Strapless Dresses Mall Gothic Skew Hem Party Mini Dress Women Grunge Style Backless Streetwear24 abril 2025
-
Netflix agora permite pagar assinatura através de cartão pré-pago - Giz Brasil24 abril 2025
-
RAZ🎟️CОMMISSIОNS ОPEN on X: Just as a reminder Ecto posts24 abril 2025
-
The costume Esdeath in'akame ga kill!24 abril 2025
-
Descargar Roblox Gratis - Descargar Roblox Gratis24 abril 2025
-
Plants vs. Zombies: Garden Warfare (PS4) - Tokyo Otaku Mode (TOM)24 abril 2025
-
O Lugar da Língua Portuguesa24 abril 2025
-
Omori self made emotion chart by EnergyNoodles on DeviantArt24 abril 2025
-
sotoru gojo edit muryo kusho dublado|TikTok Search24 abril 2025
-
Pokémon Violet e Scarlet: nada inovador, trailer revela personagens24 abril 2025