Expediting Antibody Discovery with a Cell and Bead Multiplexed

Por um escritor misterioso
Last updated 23 março 2025
Expediting Antibody Discovery with a Cell and Bead Multiplexed
A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Luminex Discovery Assays R&D Systems, a Bio-Techne Brand
Expediting Antibody Discovery with a Cell and Bead Multiplexed
iQue® Mouse IgG Type and Titer Assay - Intellicyt
Expediting Antibody Discovery with a Cell and Bead Multiplexed
What Are Immunoassays Bead-Based Immunoassays
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Multiplexed Immunoassays: Small Samples, Big Pictures
Expediting Antibody Discovery with a Cell and Bead Multiplexed
A Microfluidic Chip for Screening and Sequencing of Monoclonal
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Using Multiplexed Screening for Advanced Therapeutic Antibody
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Characterization of antibodies from the established hybridomas by
Expediting Antibody Discovery with a Cell and Bead Multiplexed
ELISA in the multiplex era: Potentials and pitfalls – topic of
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Antibody Screening Solutions Made Easy - 6
Expediting Antibody Discovery with a Cell and Bead Multiplexed
An immunogenic cell injury module for the single-cell multiplexed
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Human immunocompetent Organ-on-Chip platforms allow safety
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Multiplex Technology for Biomarker Immunoassays
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Single B-cell Antibody Development Service

© 2014-2025 progresstn.com. All rights reserved.