Assembly of recombinant tau into filaments identical to those of
Por um escritor misterioso
Last updated 29 março 2025

Many neurodegenerative diseases, including Alzheimer’s disease, the most common form of dementia, are characterised by knotted clumps of a protein called tau. In these diseases, tau misfolds, stacks together and forms abnormal filaments, which have a structured core and fuzzy coat. These sticky, misfolded proteins are thought to be toxic to brain cells, the loss of which ultimately causes problems with how people move, think, feel or behave. Reconstructing the shape of tau filaments using an atomic-level imaging technique called electron cryo-microscopy, or cryo-EM, researchers have found distinct types of tau filaments present in certain diseases. In Alzheimer’s disease, for example, a mixture of paired helical and straight filaments is found. Different tau filaments are seen again in chronic traumatic encephalopathy (CTE), a condition associated with repetitive brain trauma. It remains unclear, however, how tau folds into these distinct shapes and under what conditions it forms certain types of filaments. The role that distinct tau folds play in different diseases is also poorly understood. This is largely because researchers making tau proteins in the lab have yet to replicate the exact structure of tau filaments found in diseased brain tissue. Lövestam et al. describe the conditions for making tau filaments in the lab identical to those isolated from the brains of people who died from Alzheimer’s disease and CTE. Lövestam et al. instructed bacteria to make tau protein, optimised filament assembly conditions, including shaking time and speed, and found that bona fide filaments formed from shortened versions of tau. On cryo-EM imaging, the lab-produced filaments had the same left-handed twist and helical symmetry as filaments characteristic of Alzheimer’s disease. Adding salts, however, changed the shape of tau filaments. In the presence of sodium chloride, otherwise known as kitchen salt, tau formed filaments with a filled cavity at the core, identical to tau filaments observed in CTE. Again, this structure was confirmed on cryo-EM imaging. Being able to make tau filaments identical to those found in human tauopathies will allow scientists to study how these filaments form and elucidate what role they play in disease. Ultimately, a better understanding of tau filament formation could lead to improved diagnostics and treatments for neurodegenerative diseases involving tau.
Laboratory-based methods are presented that produce filamentous tau aggregates with the same structures as those observed in neurodegenerative disease.
Laboratory-based methods are presented that produce filamentous tau aggregates with the same structures as those observed in neurodegenerative disease.

RCSB PDB - 7QJV: In vitro assembled tau filaments into Quadruple Helical Filaments type 1 (2c)

Structure of the nonhelical filament of the Alzheimer's disease tau core

Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy

RCSB PDB - 7QL3: in vitro assembled 266/297 - 391 tau filaments with NaCl (8b)

Cryo-EM structures of tau filaments from Alzheimer's disease

Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy

Cryo-EM structure of RNA-induced tau fibrils reveals a small C-terminal core that may nucleate fibril formation

RCSB PDB - 7QKU: in vitro assembled 266/297 - 391 tau filaments with MgCl2 and NaCl (14a)

Tau filaments from amyotrophic lateral sclerosis/parkinsonism-dementia complex adopt the CTE fold

Immuno-EM of tau filaments from the frontal cortex of sporadic and

Site‐Specific Ubiquitination of Tau Amyloids Promoted by the E3 Ligase CHIP - Parolini - Angewandte Chemie International Edition - Wiley Online Library
Recomendado para você
-
297 brain test|Pesquisa do TikTok29 março 2025
-
Daze Puzzle Blog - Brain Test Level 297 (NEW) Uh! Something is29 março 2025
-
Brain Test lev.29729 março 2025
-
Brain Test 4 Level 297 We must help Uncle Bubba Answer29 março 2025
-
Escape Room: Mystery Word Level 297 Answers29 março 2025
-
Зажгите все лампы. Уровень 297 - Brain Blow29 março 2025
-
The answer to level 291, 292, 293, 294, 295, 296, 297, 298, 29929 março 2025
-
Multivariate analyses demonstrating association between obesity29 março 2025
-
Grace Hudson on X: A spot of osmosis to brighten your Wednesday29 março 2025
-
spacegodzilla1 User Profile29 março 2025
você pode gostar
-
Biggie Cheese Mr Boombastic – Calcomanía de vinilo para parachoques de automóvil – Yaxa Costa Rica29 março 2025
-
70 Desenhos da Barbie para colorir 18 Páginas para colorir, Páginas de colorir com animais, Barbie desenho29 março 2025
-
Stream episode Sony PlayStation PS2 Gaming Console 150 GB Hard Disk With 50 Games Preloaded at price below Rs.8999 by Yoshops.com podcast29 março 2025
-
STRATOVARIUS discography (top albums) and reviews29 março 2025
-
Play Stick War Legacy on PC29 março 2025
-
USED]Hades -switch ([Bonus] HADES Original soundtrack download29 março 2025
-
Knb Stories - Wattpad29 março 2025
-
GTA Liberty City Stories Mission #34 A Walk in the Park29 março 2025
-
Viking Chess Set (Individual pieces)29 março 2025
-
mr incredible spooky meme|TikTok Search29 março 2025