Cells, Free Full-Text
Por um escritor misterioso
Last updated 31 março 2025

Cancer stem cells play a crucial role in tumor initiation, metastasis, and resistance to treatment. Cellular heterogeneity and plasticity complicate the isolation of cancer stem cells. The impact of intra-tumor cellular heterogeneity using a label-free approach remains understudied in the context of treatment resistance. Here, we use the sedimentation field–flow fractionation technique to separate, without labeling, cell subpopulations of colorectal cancer cell lines and primary cultures according to their biophysical properties. One of the three sorted cell subpopulations exhibits characteristics of cancer stem cells, including high tumorigenicity in vivo and a higher frequency of tumor-initiating cells compared to the other subpopulations. Due to its chemoresistance, two- and three-dimensional in vitro chemosensitivity assays highlight the therapeutic relevance of this cancer stem cell subpopulation. Thus, our results reveal the major implication of intra-tumor cellular heterogeneity, including cancer stem cells in treatment resistance, thanks to our label-free cell sorting approach. This approach enables—by breaking down the tumor—the study the individualized response of each sorted tumor cell subpopulation and to identify chemoresistance, thus offering new perspectives for personalized therapy.
Mol Imaging Radionucl Ther on X: The Role of a Bone SPECT/CT Scan
THE LIVES OF A CELL : LEWIS THOMAS : Free Download, Borrow, and

IJMS, Free Full-Text
Cells, Free Full-Text

Cell-free mutant analysis combined with structure prediction of a

Cell-free expression and synthesis of viruses and bacteriophages

Antibodies, Free Full-Text
Cell-Free Protein Expression

Experimental design of upsidedown, upside-up, and cell-free
An illustration of the full-duplex cell-free massive MIMO system
Continuous-Flow and Continuous-Exchange Cell-Free Translation
THE LIVES OF A CELL : LEWIS THOMAS : Free Download, Borrow, and

Serial Number Alcohol 120 1.9 6 - Colaboratory

PDF) Protein structural biology using cell-free platform from
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