IJMS, Free Full-Text
Por um escritor misterioso
Last updated 25 abril 2025

Botrytis cinerea is a pathogen of wide agronomic and scientific importance partly due to its tendency to develop fungicide resistance. Recently, there has been great interest in the use of RNA interference as a control strategy against B. cinerea. In order to reduce the possible effects on non-target species, the sequence-dependent nature of RNAi can be used as an advantage to customize the design of dsRNA molecules. We selected two genes related to virulence: BcBmp1 (a MAP kinase essential for fungal pathogenesis) and BcPls1 (a tetraspanin related to appressorium penetration). After performing a prediction analysis of small interfering RNAs, dsRNAs of 344 (BcBmp1) and 413 (BcPls1) nucleotides were synthesized in vitro. We tested the effect of topical applications of dsRNAs, both in vitro by a fungal growth assay in microtiter plates and in vivo on artificially inoculated detached lettuce leaves. In both cases, topical applications of dsRNA led to gene knockdown with a delay in conidial germination for BcBmp1, an evident growth retardation for BcPls1, and a strong reduction in necrotic lesions on lettuce leaves for both genes. Furthermore, a strongly reduced expression of the BcBmp1 and BcPls1 genes was observed in both in vitro and in vivo experiments, suggesting that these genes could be promising targets for the development of RNAi-based fungicides against B. cinerea.
Ijms Free Full Text Hyaluronic Acid Influence On Normal And Osteoarthritic Tissue Engineered

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text

Ijms Free Full Text Delayed Onset Of Age Dependent Changes In Ultrastructure Of Myocardial 116100

IJMS, Free Full-Text
Ijms Free Full Text Non Invasive Detection Of Extracellular Matrix
Ijms Free Full Text Neural And Hormonal Basis Of 49680

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS Free Full-Text Research Advances In Mechanical, 52% OFF

IJMS, Free Full-Text

IJMS, Free Full-Text
Recomendado para você
-
TrucoON25 abril 2025
-
Ludo Club・Fun Dice Board Game App Price Intelligence by Qonversion25 abril 2025
-
Playing card Truco offline, others, game, online Casino, casino png25 abril 2025
-
cartoon banana peel - tilt Low-poly 3D Model25 abril 2025
-
Brachs Minions Bello Candy Corn - Banana, Blue Raspberry and Vanilla Flavored - Halloween, Harvest, Trick or Treat Corn Candy - Bulk Pack - 3 Pound25 abril 2025
-
Jogo de festa com tempero: Picante - NTT!25 abril 2025
-
Inutilidades Virtual: Jogue partidas de truco OnLine de Graça25 abril 2025
-
Go Bananas!™ Slot Machine Game to Play Free25 abril 2025
-
HOW TO FARM BANANA MACHINE 🍌Only around until new years eve so25 abril 2025
-
How to get CHROME BANANA in Tsunami Game (Roblox)25 abril 2025
você pode gostar
-
hotaru haganezuka hyotokko mask 3d print stl - kimetsu no yaiba25 abril 2025
-
Inferno 13 – Digital Dante25 abril 2025
-
One word to describe this Baldi's Basics Plus V0.2.2 run? Wacky! Here's the outro for today's video! Basically Games #mystman12 #baldisbasicsplus, By GoodKhaos25 abril 2025
-
C.P. Hart Spillo Single Lever Bidet Mixer with Pop-Up Waste, Bidet Taps25 abril 2025
-
Douma (Kimetsu no Yaiba) - Lua Superior Dois25 abril 2025
-
Best Black Friday Nintendo Switch Deals 2023 - Softonic25 abril 2025
-
Fato Pikachu bebé25 abril 2025
-
Clash of Titans on X: Strike fear in all your enemies as #Yorn shoots for nothing less than victory in #ClashOfTitans Spotted any skins of Yorn that you like best in the25 abril 2025
-
Growing Deadly Flower [NARUTO X COTE] - Chapter IV - Wattpad25 abril 2025
-
Animewallpapergifs25 abril 2025