IJMS, Free Full-Text
Por um escritor misterioso
Last updated 16 abril 2025

A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. A four-way branched oligonucleotide building block (a starlet) was designed for the assembly of the shell composed of three identical self-complementary DNA single strands and a single RNA strand for hybridization to the DNA oligonucleotides of the template. To prevent premature auto-hybridization of the self-complementary oligonucleotides in the starlet, a photolabile protecting group was introduced via the N3-substituted thymidine phosphoramidite. Cleavable linkers such as a disulfide linkage, RNase A sensitive triribonucleotides, and di- and trideoxynucleotides were incorporated into the starlet and template at specific points to guide the post-assembly disconnection of the shell from the template, and enzymatic disassembly of the template and the shell in biological media. At the same time, siRNA strands were modified with 2′-OMe ribonucleotides and phosphorothioate groups in certain positions to stabilize toward enzymatic digestion. We report herein a solid-phase synthesis of branched oligodeoxy and oligoribonucleotide building blocks for the DNA/RNA dendritic template and the branched DNA starlet for a template-assisted construction of a C24 fullerene-like DNA shell after initial molecular modeling, followed by the assembly of the shell around the DNA-coated RNA dendritic template, and visualization of the resulting nanostructure by transmission electron microscopy.

IJMS, Free Full-Text

IJMS, Free Full-Text
Ijms Free Full Text Hyaluronic Acid Influence On Normal And

IJMS Free Full-Text Research Advances In Mechanical, 52% OFF

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS Free Full-Text Immunoglobulin Abnormalities In Gaucher, 48% OFF

IJMS, Free Full-Text

IJMS, Free Full-Text

Fair Priced FavoriteIJMS, Free Full-Text, louis velasquez cgm

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text
Recomendado para você
-
O QUE É O SERVIÇO ESPECIALIZADO EM ENGENHARIA DE SEGURANÇA E16 abril 2025
-
Betye Saar The New Yorker16 abril 2025
-
Goldin 1939 DC Detective Comics #27 Auction16 abril 2025
-
78.jpg - Other Themes - CGC Comic Book Collectors Chat Boards16 abril 2025
-
Pop! Comic Cover: Marvel X-Men Wolverine PX Vinyl Figure16 abril 2025
-
Blueprint reading; a practical manual of instruction in blueprint16 abril 2025
-
CAPTAIN MARVEL #2 CGC 9.0 WHITE PAGES16 abril 2025
-
How Much Is All-Star Western #5 Worth? Browse Comic Prices16 abril 2025
-
Amazing Spider-Man #54.LR CGC 9.816 abril 2025
-
BATMAN #135 (#900) GOLD FOIL EXCLUSIVE CGC 9.816 abril 2025
você pode gostar
-
3 Types Of Brand Audits16 abril 2025
-
Como baixar Barbie Dreamhouse Adventures no Andriod16 abril 2025
-
JOGO POKÉMON SCARLET N SWITCH16 abril 2025
-
Sayer (Legacy of the Duelist)16 abril 2025
-
hope you like the music 🗿 #fyp #animan #meme16 abril 2025
-
Bernardo Corradi - Player profile16 abril 2025
-
Coleção de peças de xadrez de madeira. jogo de tabuleiro. estratégia. vetor16 abril 2025
-
No Balaio - Boteco & Empório16 abril 2025
-
Desenho e Imagem De Dentro Para Fora Amigas para Colorir e Imprimir Grátis para Adultos e Crianças16 abril 2025
-
Novo jogo de As Aventuras de Tintin está em produção16 abril 2025