Pathogens, Free Full-Text
Por um escritor misterioso
Last updated 30 março 2025

In Brazil, blood donation is regulated by the Brazilian Ministry of Health, and all States follow the same protocol for clinical and laboratory screening. Brazil is an endemic country for Chagas disease (CD), caused by Trypanosoma cruzi, and for leishmaniasis, caused by a species of Leishmania spp. Screening for leishmaniosis is not routinely performed by blood banks. Given the antigenic similarity between T. cruzi and Leishmania spp., cross-reactions in serological tests can occur, and inconclusive results for CD have been found. The objective of this study was to apply molecular techniques, e.g., nPCR, PCR, and qPCR, to clarify cases of blood donation candidates with non-negative serology for CD and to analyze the difference between the melting temperature during real-time PCR using SYBR Green. Thirty-seven cases that showed non-negative results for CD using chemiluminescent microparticle immunoassay (CMIA) tests from blood banks in Campo Grande, MS, and Campinas, SP, were analyzed. In the serum samples, 35 samples were evaluated by ELISA, and 24.3% (9/35) showed positive results for CD. nPCR was able to detect 12 positive results in 35 samples (34.28%). qPCR for T. cruzi was quantifiable in the samples that showed a value ≥0.002 par eq/mL (parasite equivalents per milliliter), and in 35 samples, 11 (31.42%) were positive. Of all evaluated samples using the described tests (CMIA, ELISA, nPCR, and qPCR), 18 (48.6%) were positive for CD. For MCA by qPCR, the melting temperature was 82.06 °C ± 0.46 for T. cruzi and 81.9 °C ± 0.24 for Leishmania infantum. The Mann–Whitney test showed a significant value of p < 0.0001. However, the differentiation between T. cruzi and L. infantum could not be considered due to temperature overlap. For leishmaniasis, of the 35 samples with non-negative serology for CD tested by the indirect fluorescent antibody test (IFAT), only one sample (2.85%) was positive (1:80). The PCR for Leishmania spp. was performed on 36 blood samples from donation candidates, and all were negative. qPCR for L. infantum showed 37 negative results for the 37 analyzed samples. The data presented here show the importance of performing two different tests in CD screening at blood banks. Molecular tests should be used for confirmation, thereby improving the blood donation system.

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix

Risks of Infectious Disease in Xenotransplantation

The influence of probiotic bacteria and human gut microorganisms causing opportunistic infections on Blastocystis ST3, Gut Pathogens

Pathogens, Free Full-Text

Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis - The Lancet

Bloodborne Pathogens Certificate Templates 6 FREE Designs

Evolution of pathogen tolerance and emerging infections: A missing experimental paradigm

Free Google Slides and PowerPoint templates about bacteria

Disarming the pathogen Nature Chemical Biology

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix
ThinkLite Natick MA

Pathogens and Infectious Disease Word Search - WordMint

Pathogens, Free Full-Text
Bacterial Fish Pathogens: Disease of Farmed and Wild Fish
Recomendado para você
-
Colégio Pedro II30 março 2025
-
Ler ou não ler o boletim Focus? Eis a questão - Inteligência Financeira30 março 2025
-
Omphalocele as component of multiple anomaly syndrome/sequence30 março 2025
-
Dólar hoje: moeda reage às expectativas do Boletim Focus no Brasil – Últimas notícias – Estadão E-Investidor – As principais notícias do mercado financeiro30 março 2025
-
Size and crown shape predict reproductive maturity of Carapa guianensis in upland and floodplain forests in the northeastern - Angulo Villacorta - Biotropica - Wiley Online Library30 março 2025
-
SILICONE NEUTRO ULTIMATE MF CHEMISIL – Chemitool30 março 2025
-
GazetaWeb - Boletim mostra queda da taxa de letalidade por covid-19 no Rio30 março 2025
-
A review of exotic Kalanchoe taxa (Crassulaceae subfam. Kalanchooideae) naturalised and cultivated in Africa and the Gulf of Guinea islands, with their nomenclature updated30 março 2025
-
Fazenda reduz previsão de alta do PIB de 2023 de 3,2% para 3%; projeção é maior que do mercado30 março 2025
-
BG - Deciphering the origin of dubiofossils from the Pennsylvanian of the Paraná Basin, Brazil30 março 2025
você pode gostar
-
Goth Anime Boy Gothic Japanese Monster Vaporware Aesthetic - Goth - Pin30 março 2025
-
Seishun Buta Yarou wa Bunny Girl Senpai no Yume wo Minai Image by30 março 2025
-
one piece - Cover Art Market30 março 2025
-
What percentile of fide 1600 rated player? - Chess Forums30 março 2025
-
Online Board Game Store – The Raving Gamer30 março 2025
-
Hotdog Coreano - Cachorro Quente no Palito - Receitas de Minuto30 março 2025
-
Alien vs. Predator: Armageddon ebook by Tim Lebbon - Rakuten Kobo30 março 2025
-
Assassin's Creed 3 - All Missions30 março 2025
-
Chief People Hacker at X-Force Red, Stephanie Snow Carruthers uses h30 março 2025
-
Night Shift: What It Is and How It Works — Allgaier Consulting, LLC30 março 2025