Pathogens, Free Full-Text
Por um escritor misterioso
Last updated 24 março 2025

In Brazil, blood donation is regulated by the Brazilian Ministry of Health, and all States follow the same protocol for clinical and laboratory screening. Brazil is an endemic country for Chagas disease (CD), caused by Trypanosoma cruzi, and for leishmaniasis, caused by a species of Leishmania spp. Screening for leishmaniosis is not routinely performed by blood banks. Given the antigenic similarity between T. cruzi and Leishmania spp., cross-reactions in serological tests can occur, and inconclusive results for CD have been found. The objective of this study was to apply molecular techniques, e.g., nPCR, PCR, and qPCR, to clarify cases of blood donation candidates with non-negative serology for CD and to analyze the difference between the melting temperature during real-time PCR using SYBR Green. Thirty-seven cases that showed non-negative results for CD using chemiluminescent microparticle immunoassay (CMIA) tests from blood banks in Campo Grande, MS, and Campinas, SP, were analyzed. In the serum samples, 35 samples were evaluated by ELISA, and 24.3% (9/35) showed positive results for CD. nPCR was able to detect 12 positive results in 35 samples (34.28%). qPCR for T. cruzi was quantifiable in the samples that showed a value ≥0.002 par eq/mL (parasite equivalents per milliliter), and in 35 samples, 11 (31.42%) were positive. Of all evaluated samples using the described tests (CMIA, ELISA, nPCR, and qPCR), 18 (48.6%) were positive for CD. For MCA by qPCR, the melting temperature was 82.06 °C ± 0.46 for T. cruzi and 81.9 °C ± 0.24 for Leishmania infantum. The Mann–Whitney test showed a significant value of p < 0.0001. However, the differentiation between T. cruzi and L. infantum could not be considered due to temperature overlap. For leishmaniasis, of the 35 samples with non-negative serology for CD tested by the indirect fluorescent antibody test (IFAT), only one sample (2.85%) was positive (1:80). The PCR for Leishmania spp. was performed on 36 blood samples from donation candidates, and all were negative. qPCR for L. infantum showed 37 negative results for the 37 analyzed samples. The data presented here show the importance of performing two different tests in CD screening at blood banks. Molecular tests should be used for confirmation, thereby improving the blood donation system.

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix

Risks of Infectious Disease in Xenotransplantation

The influence of probiotic bacteria and human gut microorganisms causing opportunistic infections on Blastocystis ST3, Gut Pathogens

Pathogens, Free Full-Text

Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis - The Lancet

Bloodborne Pathogens Certificate Templates 6 FREE Designs

Evolution of pathogen tolerance and emerging infections: A missing experimental paradigm

Free Google Slides and PowerPoint templates about bacteria

Disarming the pathogen Nature Chemical Biology

A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix
ThinkLite Natick MA

Pathogens and Infectious Disease Word Search - WordMint

Pathogens, Free Full-Text
Bacterial Fish Pathogens: Disease of Farmed and Wild Fish
Recomendado para você
-
gente o que significa situação 1, 4 e o - no boletim escolar24 março 2025
-
Boletim Epidemiológico (01/04/2022) - Prefeitura Municipal de Palestina do Pará24 março 2025
-
Boletim online. Confira como funciona! – Forleven24 março 2025
-
Boletim OBJO - Ordem - Federação Ornitológica do Brasil24 março 2025
-
Caso de Covid-19 é descartado em Pedro Osório24 março 2025
-
Ouvidoria do MF anuncia novos canais de atendimento — Ministério da Fazenda24 março 2025
-
T8986 - Boletim Informativo FENASEG - 238/260_1974 by CNseg - Issuu24 março 2025
-
T8989 - Boletim Informativo FENASEG - 307/324_1975 by CNseg - Issuu24 março 2025
-
Mudanças no texto do Decreto 11.545 e cumprimento da Portaria MF 727 ou deflagração da greve - Sindifisco Nacional24 março 2025
-
CALEFATOR LAREIRA MF A LENHA ADATTO 90CM COM MOLDURA 4 LADOS E VENTILADOR24 março 2025
você pode gostar
-
Children's Posters Naruto, Naruto shippuden, Anime naruto24 março 2025
-
REVIEW] Resident Evil: Decades of Horror (Village Edition) - Aug 2023 – Humble Bundle24 março 2025
-
Escape Simulator - Wikipedia24 março 2025
-
Friso Roda Moto Honda Cg Bros Xre Biz Pop Pcx Personalizado24 março 2025
-
Searching for The Real Jean Laffite24 março 2025
-
Nerf N-Strike Elite Hail-Fire Blaster24 março 2025
-
When your a Rich Noob (meme) ROBLOX24 março 2025
-
21 ideias de Capivara ❤ capivara, capivaras, fotos de capivara24 março 2025
-
10+ Desenhos do PK XD para Imprimir e Colorir/Pintar24 março 2025
-
Download do aplicativo Uno Offline 2023 - Grátis - 9Apps24 março 2025