Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
Por um escritor misterioso
Last updated 16 abril 2025
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-5-full.png)
Background Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease with persistent synovitis. In the present study, the impact of monocyte chemoattractant protein-1 (MCP-1) was explored to determine methods for the diagnosis and treatment of RA. Methods First, fibroblast-like synoviocytes (FLSs) were obtained from a collagen-induced rat RA model. Next, MCP-1-overexpression plasmid and small interfering RNA were transfected into human and rat FLSs. Cell Counting Kit-8 (CCK-8), Transwell migration and flow cytometry assays were used to analyze cell proliferation, migration and apoptosis of FLSs following MCP-1 transfections, respectively. Furthermore, western blotting was used to analyze the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β in FLSs following MCP-1 transfection. In addition, reverse transcription-quantitative PCR and ELISAs were used to analyze the expression levels of C-reactive protein (CRP), estrogen receptor, MCP-1 and pentraxin-3 in patients with clinical RA, followed by correlation analysis of clinical data. Finally, expression validation, diagnostic and protein-protein interaction (PPI) network analysis of MCP-1 were performed. Results MCP-1 promoted FLS proliferation and migration, and affected the apoptosis of FLSs. In addition, the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β were also affected by MCP-1. In patients with clinical RA, the expression level of MCP-1 was increased. Moreover, CRP expression level was significantly up-regulated in RA. Clinically, MCP-1 was strongly correlated with tender joint count, swollen joint count, visual analog scale for general health and disease activity score 28 (DAS28)-MCP-1, and was moderately correlated with DAS28 and DAS28-CRP. PPI analysis showed that MCP-1 mainly interacted with other inflammatory cytokines. Conclusion In conclusion, MCP-1 may play a significant regulatory role in RA, and could be used as a measurement index of clinical RA activity.
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://europepmc.org/articles/PMC7226834/bin/cells-09-00880-g006.jpg)
Update on the Pathomechanism, Diagnosis, and Treatment Options for Rheumatoid Arthritis. - Abstract - Europe PMC
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0753332222002359-gr2.jpg)
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![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://europepmc.org/articles/PMC7226834/bin/cells-09-00880-g003.jpg)
Update on the Pathomechanism, Diagnosis, and Treatment Options for Rheumatoid Arthritis. - Abstract - Europe PMC
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://pubs.acs.org/cms/10.1021/acsptsci.9b00093/asset/images/large/pt9b00093_0006.jpeg)
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![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://pub.mdpi-res.com/cells/cells-09-00880/article_deploy/html/images/cells-09-00880-g005.png?1602181481)
Cells, Free Full-Text
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.frontiersin.org/files/Articles/922111/fimmu-13-922111-HTML-r1/image_m/fimmu-13-922111-g006.jpg)
Frontiers Two Main Cellular Components in Rheumatoid Arthritis: Communication Between T Cells and Fibroblast-Like Synoviocytes in the Joint Synovium
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.frontiersin.org/files/Articles/376453/fimmu-09-01467-HTML/image_m/fimmu-09-01467-g005.jpg)
Frontiers Ontology and Function of Fibroblast-Like and Macrophage-Like Synoviocytes: How Do They Talk to Each Other and Can They Be Targeted for Rheumatoid Arthritis Therapy?
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0006295219301157-gr1.jpg)
The many facets of macrophages in rheumatoid arthritis - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/Revision_Supplementary_Figure_1.png)
Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs13075-017-1303-3/MediaObjects/13075_2017_1303_Fig2_HTML.gif)
Fibroblast-like synoviocyte metabolism in the pathogenesis of rheumatoid arthritis, Arthritis Research & Therapy
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.frontiersin.org/files/Articles/472474/fimmu-10-01743-HTML-r1/image_m/fimmu-10-01743-g001.jpg)
Frontiers Fibroblast-Like Synoviocytes Glucose Metabolism as a Therapeutic Target in Rheumatoid Arthritis
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41392-020-00443-w/MediaObjects/41392_2020_443_Fig1_HTML.png)
Metabolism pathways of arachidonic acids: mechanisms and potential therapeutic targets
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.mdpi.com/ijms/ijms-23-13016/article_deploy/html/images/ijms-23-13016-g001.png)
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