SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]
Por um escritor misterioso
Last updated 29 março 2025
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2023/15077/1/fig-1-full.png)
Understanding the interactions between SARS-CoV-2 and host cell machinery may reveal new targets to treat COVID-19. We focused on an interaction between the SARS-CoV-2 ORF3A accessory protein and the CLIC-like chloride channel-1 (CLCC1). We found that ORF3A partially co-localized with CLCC1 and that ORF3A and CLCC1 could be co-immunoprecipitated. Since CLCC1 plays a role in the unfolded protein response (UPR), we hypothesized that ORF3A may also play a role in the UPR. Indeed, ORF3A expression triggered a transcriptional UPR that was similar to knockdown of CLCC1. ORF3A expression in 293T cells induced cell death and this was rescued by the chemical chaperone taurodeoxycholic acid (TUDCA). Cells with CLCC1 knockdown were partially protected from ORF3A-mediated cell death. CLCC1 knockdown upregulated several of the homeostatic UPR targets induced by ORF3A expression, including HSPA6 and spliced XBP1, and these were not further upregulated by ORF3A. Our data suggest a model where CLCC1 silencing triggers a homeostatic UPR that prevents cell death due to ORF3A expression.
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2023/15077/1/Uncroppedwesternblots.png)
SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://media.cellsignal.com/product/image/45596_fig08_IHC-P_280_20210430193151.png)
PDI (E7O2R) Mouse mAb Cell Signaling Technology
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://journals.asm.org/cms/10.1128/mbio.03030-23/asset/eda253a0-6187-4dd8-b8b5-9c9d14803a15/assets/images/large/mbio.03030-23.f004.jpg)
Endoplasmic reticulum-associated SARS-CoV-2 ORF3a elicits heightened cytopathic effects despite robust ER-associated degradation
Hannah Gruner - Postdoctoral Fellow - Swarthmore College
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://iiif.elifesciences.org/lax:84477%2Felife-84477-fig5-v3.tif/full/1500,/0/default.jpg)
The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g002.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://iiif.elifesciences.org/lax:84477%2Felife-84477-fig4-v3.tif/full/1500,/0/default.jpg)
The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.science.org/cms/10.1126/science.1232033/asset/bdc3c9c9-2e9d-49c2-a830-548d5bd71f1e/assets/graphic/339_823_f1.jpeg)
RNA-Guided Human Genome Engineering via Cas9
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://d2jx2rerrg6sh3.cloudfront.net/image-handler/picture/2020/8/%402020.08.18.256776v1.jpg)
SARS-CoV-2 ORF9c responsible for immune evasion and efficient infection of host
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g003.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S2213231722001604-ga1.jpg)
SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases - ScienceDirect
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g004.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
Recomendado para você
-
Play Real Love Tester Online - Free Browser Games29 março 2025
-
john and brooke skipworth|TikTok Search29 março 2025
-
kogo zrobić????29 março 2025
-
Emma Brown (@EmmaBrownEDU) / X29 março 2025
-
DOWNLOAD]-Go To College For Free: College Planning ABC's Guide To Finding Scholarships, Financial Aid and Free Tuition Awards For Co…, by Jessicajones29 março 2025
-
Sequence alignment of the C-and A-sheet domains of the lamprey LV29 março 2025
-
Sonoma County Virtual Israeli Film Festival29 março 2025
-
Learn Godot 4 by Making a 2D Platformer — Part 14: Lives, Score, & Attack Boosts #2 - DEV Community29 março 2025
-
Fashion Nail Salon - Jogo Gratuito Online29 março 2025
-
Nurofen owner Reckitt Benckiser ordered to stop selling 'misleading' target specific painkillers, The Independent29 março 2025
você pode gostar
-
GTA RP: Paulinho o Loko se torna governador do Complexo e coloca o GTA RP de cabeça pra baixo - SBT29 março 2025
-
Louco pelo Corinthians - CORINTHIANS DE TODOS OS TEMPOS!! VOTAÇÃO29 março 2025
-
Potato Sack Racing Simulator 2024 : Digital Deluxe Mega Chad Edition29 março 2025
-
Movie Pretty Cure All Stars F – film comic – Japanese Creative29 março 2025
-
Tales of the Jedi (TV Series 2022– ) - IMDb29 março 2025
-
Preorder the Game to Grow book! - Game to Grow29 março 2025
-
Giroud perde pênalti, mas garante classificação do Milan sobre o29 março 2025
-
Piccolo (Dragonball Evolution), Wiki Dynami Battles29 março 2025
-
Paradise Falls, Hiking route in North Carolina29 março 2025
-
Eclipse Community Forums: PHP Development Tools (PDT) » Generate Methods in PHP class29 março 2025