SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]
Por um escritor misterioso
Last updated 31 março 2025
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2023/15077/1/fig-1-full.png)
Understanding the interactions between SARS-CoV-2 and host cell machinery may reveal new targets to treat COVID-19. We focused on an interaction between the SARS-CoV-2 ORF3A accessory protein and the CLIC-like chloride channel-1 (CLCC1). We found that ORF3A partially co-localized with CLCC1 and that ORF3A and CLCC1 could be co-immunoprecipitated. Since CLCC1 plays a role in the unfolded protein response (UPR), we hypothesized that ORF3A may also play a role in the UPR. Indeed, ORF3A expression triggered a transcriptional UPR that was similar to knockdown of CLCC1. ORF3A expression in 293T cells induced cell death and this was rescued by the chemical chaperone taurodeoxycholic acid (TUDCA). Cells with CLCC1 knockdown were partially protected from ORF3A-mediated cell death. CLCC1 knockdown upregulated several of the homeostatic UPR targets induced by ORF3A expression, including HSPA6 and spliced XBP1, and these were not further upregulated by ORF3A. Our data suggest a model where CLCC1 silencing triggers a homeostatic UPR that prevents cell death due to ORF3A expression.
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2023/15077/1/Uncroppedwesternblots.png)
SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://media.cellsignal.com/product/image/45596_fig08_IHC-P_280_20210430193151.png)
PDI (E7O2R) Mouse mAb Cell Signaling Technology
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://journals.asm.org/cms/10.1128/mbio.03030-23/asset/eda253a0-6187-4dd8-b8b5-9c9d14803a15/assets/images/large/mbio.03030-23.f004.jpg)
Endoplasmic reticulum-associated SARS-CoV-2 ORF3a elicits heightened cytopathic effects despite robust ER-associated degradation
Hannah Gruner - Postdoctoral Fellow - Swarthmore College
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://iiif.elifesciences.org/lax:84477%2Felife-84477-fig5-v3.tif/full/1500,/0/default.jpg)
The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g002.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://iiif.elifesciences.org/lax:84477%2Felife-84477-fig4-v3.tif/full/1500,/0/default.jpg)
The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.science.org/cms/10.1126/science.1232033/asset/bdc3c9c9-2e9d-49c2-a830-548d5bd71f1e/assets/graphic/339_823_f1.jpeg)
RNA-Guided Human Genome Engineering via Cas9
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://d2jx2rerrg6sh3.cloudfront.net/image-handler/picture/2020/8/%402020.08.18.256776v1.jpg)
SARS-CoV-2 ORF9c responsible for immune evasion and efficient infection of host
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g003.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S2213231722001604-ga1.jpg)
SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases - ScienceDirect
![SARS-CoV-2 ORF3A interacts with the Clic-like chloride channel-1 (CLCC1) and triggers an unfolded protein response [PeerJ]](https://www.frontiersin.org/files/Articles/854567/fmicb-13-854567-HTML/image_m/fmicb-13-854567-g004.jpg)
Frontiers Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
Recomendado para você
-
My Dream Hospital - Free Play & No Download31 março 2025
-
LOLOI II Grand Canyon Grey/Ivory 6 ft. 2 in. x 8 ft. Transitional Area Rug GRANGC-11GYIV6280 - The Home Depot31 março 2025
-
Love Tester - Free Online Mobile Game on31 março 2025
-
The False Prophet (Stonegate)31 março 2025
-
kogo zrobić????31 março 2025
-
Hannah Rosenberg ⚡ (@hmichellerose) / X31 março 2025
-
CapCut_zild at yiv31 março 2025
-
Announcing Flutter for Windows - Google for Developers31 março 2025
-
Piwaka Hug Blanket Gifts for Loved One - Cosy Sherpa Fleece Blanket in Grey, Machine Washable Plush Blankets - Heartwarming Gifts, Sentimental Gifts, Get Well Soon Gifts for Women31 março 2025
-
Corrin+Aqua+Mona - Ocean's Grey Waves by julianx2rl on DeviantArt31 março 2025
você pode gostar
-
July 11, 2010 - Johannesburg, South Africa - Gregory Van Der Wiel31 março 2025
-
how to get every item in king legacy|TikTok Search31 março 2025
-
Pin de Anzjdb em Cualquier Weea, Personagens de anime, Cabelo masculino anime, Animes wallpapers31 março 2025
-
LEGO® Star Wars™ The Last Jedi Resistance Transport Pod™ 75176 - A & D Products NY Corp. Cool Toy Den31 março 2025
-
Serie bkt logo hi-res stock photography and images - Alamy31 março 2025
-
Petition · Classroom Of The Elite Season 2 ·31 março 2025
-
COMO HACER UNA TORTA DE POU?31 março 2025
-
NBA's Greatest 75 Players: Ranking the top 10 peaks in NBA history31 março 2025
-
5x5 TicTacToe Toss Carnival Game Rental31 março 2025
-
Ro-Ghoul staff team expose!31 março 2025